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Uptake and dissimilation of glycerol by wild type and glycerol nonutilizing strains of Neurospora crassa

✍ Scribed by Pyle, Joseph E. ;Howe, H. Branch

Publisher: Springer
Year: 1983
Tongue: English
Weight: 327 KB
Volume: 189
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00326071

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✦ Synopsis

Seven mutant strains defective for utilization of glycerol, glyceraldehyde or dihydroxyacetone were isolated. One strain was deficient for NAD-linked glycerol-3-phosphate dehydrogenase, two for glycerol kinase, and four had no detected enzymatic deficiency, although one of the latter strains was deficient in glycerol uptake. Glycerol uptake was increased by incubation in glycerol, glycerol-3-phosphate, erythritol, and propanediol, and was protein-mediated below 0.14 mM glycerol, but at higher concentrations free diffusion predominated. Glycerol uptake was decreased by cycloheximide and was more sensitive to sodium azide than to iodoacetate.

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