## Abstract 3T3 mouse fibroblasts in 10% calf serum cease to increase in number at confluence. SV40‐transformed 3T3 cells lose their sensitivity to this growth control and so are able to reach multilayered cell densities. We previously described a polyploid revertant line F1SV101, isolated from a S
The Isolation of Tubulin and Actin from Mouse 3T3 Cells Transformed by Simian Virus 40 (SV3T3 Cells), an Established Cell Line Growing in Culture
✍ Scribed by K. WEBER; R. KOCH; W. HERZOG; J. VANDEKERCKHOVE
- Book ID
- 115116161
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 640 KB
- Volume
- 78
- Category
- Article
- ISSN
- 1432-1327
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## Abstract SV101, the SV40‐transformed subline of the mouse fibroblast line 3T3, is both serum‐ and density transformed, since it grows in both 1% and 10% calf serum, and grows beyond confluence in 10% calf serum. Negative selection at low cell density in 1% calf serum or in 10% agamma‐depleted se
## Abstract The growth requirements of SV40 transformed Balb/c‐3T3 cells have been studied in the absence of serum. For growth in serum‐free medium, the cells require (i) insulin, (ii) transferrin, and (iii) cis‐unsaturated fatty acids added in combination with fatty acid free bovine serum albumin.
## Abstract Cell strains isolated from an established line of SV40‐transformed mouse 3T3 cells (SV3T3) showed large variations in the various parameters of transformation, viz. saturation density, serum requirement, contact inhibition of movement and of growth and Concanavalin‐A‐mediated agglutinab