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The influence of DNA binding on the backbone dynamics of the yeast cell-cycle protein Mbp1*

โœ Scribed by Pauline B. McIntosh; Ian A. Taylor; Thomas A. Frenkiel; Stephen J. Smerdon; Andrew N. Lane


Book ID
110263154
Publisher
Springer Netherlands
Year
2000
Tongue
English
Weight
186 KB
Volume
16
Category
Article
ISSN
0925-2738

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โœ Ian A. Taylor; Stephen J. Smerdon ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 31 KB ๐Ÿ‘ 2 views

A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant