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The X-ray structure of the DNA-binding domain from the Saccharomyces cerevisiae cell-cycle transcription factor Mbp1 at 2.1 Å resolution

✍ Scribed by Ian A Taylor; Monika K Treiber; Luca Olivi; Stephen J Smerdon


Book ID
115628094
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
448 KB
Volume
272
Category
Article
ISSN
0022-2836

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✍ Ian A. Taylor; Stephen J. Smerdon 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 31 KB 👁 2 views

A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant