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Characterization of the DNA-Binding Domains from the Yeast Cell-Cycle Transcription Factors Mbp1 and Swi4

โœ Scribed by Taylor, Ian A.; McIntosh, Pauline B.; Pala, Preshna; Treiber, Monika K.; Howell, Steven; Lane, Andrew N.; Smerdon, Stephen J.


Book ID
126176281
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
479 KB
Volume
39
Category
Article
ISSN
0006-2960

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Expression, purification, and crystalliz
โœ Ian A. Taylor; Stephen J. Smerdon ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 31 KB ๐Ÿ‘ 2 views

A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant