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Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis

โœ Scribed by Rui-Ming Xu; Christian Koch; Yu Liu; John R Horton; Dunja Knapp; Kim Nasmyth; Xiaodong Cheng


Book ID
114329470
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
916 KB
Volume
5
Category
Article
ISSN
0969-2126

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Expression, purification, and crystalliz
โœ Ian A. Taylor; Stephen J. Smerdon ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 31 KB ๐Ÿ‘ 2 views

A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. Crystals have been grown using PEG 3350 as precipitant