𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The inducible expression of the long pentraxin PTX3 in osteoblast-like MC 3T3-E1 cells by E. coli lipopolysaccharide

✍ Scribed by Binbin Liu; Yin-kun Liu; Atsuko Nemoto; Toshimasa Uemura

Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
167 KB
Volume
24
Category
Article
ISSN
0928-4931

No coin nor oath required. For personal study only.

✦ Synopsis

In this study, we have found that Escherichia coli lipopolysaccharide (LPS) could induce the expression of pentraxin 3 (PTX3) mRNA in osteoblast-like cells (MC 3T3-E1) dose dependently. The relation between this expression and alkaline phosphatase (ALP), mitogen-activated protein kinases (MAPKs), and nuclear factor kappa B (NF-nB) was also analyzed. The results show that the mRNA expression of PTX3 was related to that of ALP. It was also related to the mRNA expression of ERK-1 and p38 but not JNK-1 and NF-nB. These results suggest that ERK-1 and p38 are involved in the regulation of PTX3 expression and PTX3 promotes the differentiation of MC 3T3-E1 cells in response to LPS.

📜 SIMILAR VOLUMES

Constitutive expression of thrombospondi
Constitutive expression of thrombospondin 1 in MC3T3-E1 osteoblastic cells inhibits mineralization
✍ Akemichi Ueno; Yoshihiro Miwa; Keiko Miyoshi; Taigo Horiguchi; Hideo Inoue; Inta 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 788 KB

## Abstract Thrombospondin 1 (TSP1) is a multifunctional extracellular glycoprotein present mainly in the fetal and adult skeleton. Although an inhibitory effect of TSP1 against pathological mineralization in cultured vascular pericytes has been shown, its involvement in physiological mineralizatio

Effects of sintered bovine bone on cell
Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3-E1 osteoblast-like cells
✍ Takuji Matsumoto; Mamoru Kawakami; Koichi Kuribayashi; Toru Takenaka; Akihito Mi 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 745 KB

## Abstract To determine the function of biomaterials on osteoblasts, we studied the effects of two different biomaterials, sintered bovine bone (true bone ceramic) and hydroxyapatite‐related material, on DNA and collagen synthesis and osteoblastic expression in osteoblast‐like cells __in vitro__.

Long-term loading inhibits ERK1/2 phosph
Long-term loading inhibits ERK1/2 phosphorylation and increases FGFR3 expression in MC3T3-E1 osteoblast cells
✍ Rebecca A. Jackson; Arjuna Kumarasuriyar; Victor Nurcombe; Simon M. Cool 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 340 KB

## Abstract Bone tissue homeostasis relies upon the ability of cells to detect and interpret extracellular signals that direct changes in tissue architecture. This study utilized a four‐point bending model to create both fluid shear and strain forces (loading) during the time‐dependent progression

Induction of osteoblast differentiation
Induction of osteoblast differentiation indices by statins in MC3T3-E1 cells
✍ Toyonobu Maeda; Ayako Matsunuma; Izuru Kurahashi; Toru Yanagawa; Hiroshi Yoshida 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB 👁 1 views

## Abstract Statins inhibit 3‐hydroxy‐3‐methylglutaryl‐coenzyme A (HMG‐CoA) reductase, which catalyzes conversion of HMG‐CoA to mevalonate, a rate‐limiting step in cholesterol synthesis. The present study was undertaken to understand the events of osteoblast differentiation induced by statins. Simv