𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction of osteoblast differentiation indices by statins in MC3T3-E1 cells

✍ Scribed by Toyonobu Maeda; Ayako Matsunuma; Izuru Kurahashi; Toru Yanagawa; Hiroshi Yoshida; Noboru Horiuchi

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
261 KB
Volume
92
Category
Article
ISSN
0730-2312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Statins inhibit 3‐hydroxy‐3‐methylglutaryl‐coenzyme A (HMG‐CoA) reductase, which catalyzes conversion of HMG‐CoA to mevalonate, a rate‐limiting step in cholesterol synthesis. The present study was undertaken to understand the events of osteoblast differentiation induced by statins. Simvastatin at 10^−7^ M markedly increased mRNA expression for bone morphogenetic protein‐2 (BMP‐2), vascular endothelial growth factor (VEGF), alkaline phosphatase, type I collagen, bone sialoprotein, and osteocalcin (OCN) in nontransformed osteoblastic cells (MC3T3‐E1), while suppressing gene expression for collagenase‐1, and collagenase‐3. Extracellular accumulation of proteins such as VEGF, OCN, collagenase‐digestive proteins, and noncollagenous proteins was increased in the cells treated with 10^−7^ M simvastatin, or 10^−8^ M cerivastatin. In the culture of MC3T3‐E1 cells, statins stimulated mineralization; pretreating MC3T3‐E1 cells with mevalonate, or geranylgeranyl pyrophosphate (a mevalonate metabolite) abolished statin‐induced mineralization. Statins stimulate osteoblast differentiation in vitro, and may hold promise drugs for the treatment of osteoporosis in the future. © 2004 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Regulation of osteoblast differentiation
Regulation of osteoblast differentiation by Nurr1 in MC3T3-E1 cell line and mouse calvarial osteoblasts
✍ Mi Kyeong Lee; Hosoon Choi; Minchan Gil; Vera M. Nikodem 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 170 KB 👁 1 views

## Abstract The orphan nuclear receptor Nurr1 is primarily expressed in the central nervous system. It has been shown that Nurr1 is necessary for terminal differentiation of dopaminergic (DA) neurons in ventral midbrain. The receptor, however, is also expressed in other organs including bone, even

Polymethylmethacrylate particles inhibit
Polymethylmethacrylate particles inhibit osteoblastic differentiation of MC3T3-E1 osteoprogenitor cells
✍ Richard Chiu; Ting Ma; R. Lane Smith; Stuart B. Goodman 📂 Article 📅 2008 🏛 Elsevier Science 🌐 English ⚖ 250 KB

## Abstract Orthopedic wear debris has been implicated as a significant inhibitory factor of osteoblast differentiation. Polymethylmethacrylate (PMMA) particles have been previously shown to inhibit the differentiation of osteoprogenitors in heterogeneous murine marrow stromal cell cultures, but th

β-cryptoxanthin stimulates cell differen
β-cryptoxanthin stimulates cell differentiation and mineralization in osteoblastic MC3T3-E1 cells
✍ Satoshi Uchiyama; Masayoshi Yamaguchi 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 431 KB

## Abstract The effect of β‐cryptoxanthin, a kind of carotenoid, on cell differentiation and mineralization in osteoblastic MC3T3‐E1 cells was investigated. Cells were cultured for 72 h in a minimum essential medium containing 10% fetal bovine serum (FBS), and the cells with subconfluency were chan

Suppressive effect of regucalcin on cell
Suppressive effect of regucalcin on cell differentiation and mineralization in osteoblastic MC3T3-E1 cells
✍ Masayoshi Yamaguchi; Mayumi Kobayashi; Satoshi Uchiyama 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 301 KB 👁 2 views

## Abstract The role of regucalcin in the regulation of osteoblastic cell function was investigated. Osteoblastic MC3T3‐E1 cells with subconfluent monolayers were cultured in a medium containing regucalcin (10^−10^–10^−8^ M) without fetal bovine serum (FBS). The proliferation of osteoblastic cells

Hydroxyapatite accelerates differentiati
Hydroxyapatite accelerates differentiation and suppresses growth of MC3T3-E1 osteoblasts
✍ Shu, R. ;McMullen, R. ;Baumann, M. J. ;McCabe, L. R. 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 224 KB

## Abstract Hydroxyapatite describes both the natural mineral phase of bone as well as the widely used calcium–phosphate implant substitute. Given that hydroxyapatite is a major component of the __in vivo__ surface with which osteoblasts interact, it is surprising that most studies examining the re

Rapamycin inhibits osteoblast proliferat
Rapamycin inhibits osteoblast proliferation and differentiation in MC3T3-E1 cells and primary mouse bone marrow stromal cells
✍ Ujjal K. Singha; Yu Jiang; Shibing Yu; Min Luo; Yi Lu; Jian Zhang; Guozhi Xiao 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 298 KB 👁 1 views

## Abstract While the roles of the mammalian target of rapamycin (mTOR) signaling in regulation of cell growth, proliferation, and survival have been well documented in various cell types, its actions in osteoblasts are poorly understood. In this study, we determined the effects of rapamycin, a spe