✦ LIBER ✦

Effects of sintered bovine bone on cell proliferation, collagen synthesis, and osteoblastic expression in MC3T3-E1 osteoblast-like cells

✍ Scribed by Takuji Matsumoto; Mamoru Kawakami; Koichi Kuribayashi; Toru Takenaka; Akihito Minamide; Tetsuya Tamaki

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 745 KB
Volume: 17
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100170419

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

To determine the function of biomaterials on osteoblasts, we studied the effects of two different biomaterials, sintered bovine bone (true bone ceramic) and hydroxyapatite‐related material, on DNA and collagen synthesis and osteoblastic expression in osteoblast‐like cells in vitro. Osteoblasts cultured with true bone ceramic exhibited greater increases in DNA and collagenous protein synthesis and alkaline phosphatase activity than those cultured with hydroxyapatite. Furthermore, expression of mRNA for type‐I collagen and osteocalcin, as assessed by reverse transcription‐polymerase chain reaction, was higher in cultures grown on true bone ceramic or hydroxyapatite than in those grown with glass fragments, and levels of gene expression in true bone ceramic and hydroxyapatite cultures were at almost the same level. These findings indicate that osteoblasts cultured with true bone ceramic increase their activity, suggesting that true bone ceramic may be a more favorable substrate than hydroxyapatite for growth and differentiation of osteoblast‐like cells.

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