𝔖 Bobbio Scriptorium
✦   LIBER   ✦

The incorporation of microinjected 14C-amino acids into TCA insoluble fractions of the giant axon of the squid

✍ Scribed by S. Fischer; S. Litvak

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
436 KB
Volume
70
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Giant squid axons were microinjected with serine, valine and leucine-C14 under controlled electrophysiological conditions. These amino acids are incorporated into TCA insoluble fraction in the isolated axon.

This incorporation is higher in the stimulated axons as compared to non-stimulated ones. By processing separately the axoplasm and axon sheath, it was found that the last one is responsible almost entirely for the observed incorporation.

Through differential centrifugation of homogenates of microinjected axons was shown that the highest incorporation occurred in the 1500 x g sediment, which probably corresponds to membranes. The incorporation of amino acids in stimulated axons, is strongly inhibited by chloramphenicol and actinomycin D.

πŸ“œ SIMILAR VOLUMES

Incorporation of 2(S)-methylbutanoic aci
Incorporation of 2(S)-methylbutanoic acid-1-14C into the structure of mevinolin
✍ Alvin K. Willard; Robert L. Smith πŸ“‚ Article πŸ“… 1982 πŸ› John Wiley and Sons 🌐 French βš– 334 KB

## Abstract A route to mevinolin () bearing (S)‐2‐methylbutanoic acid ‐1‐^14^C as the ester side chain has been developed. The structure of mevinolin () was degraded and selectively protected to provide the alcohol in three steps. Incorporation of ^14^C was accomplished by acylation of alcohol with

Biosynthesis of crustacean lipovitellin.
Biosynthesis of crustacean lipovitellin. III. The incorporation of labeled amino acids into the purified lipovitellin of the crabPachygrapsus crassipes
✍ Lui, Chung W. ;O'Connor, John D. πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 355 KB πŸ‘ 1 views

## Abstract Electrophoretically pure subunits were obtained from the lipovitellin (LV) of the intertidal crab, __Pachygrapsus crassipes__. The molecular weights of these subunits were determined. In addition, it was demonstrated that the ovaries of __Pachygrapsus__, incubated __in vitro__, incorpor

Energy requirement for the incorporation
Energy requirement for the incorporation of amino acids into protein of isolated mouse-liver mitochondria
✍ Patrick F. Delaney; Norman J. Girardot πŸ“‚ Article πŸ“… 1966 πŸ› John Wiley and Sons 🌐 English βš– 238 KB πŸ‘ 1 views

Mitochondria isolated from mouse liver can incorporate amino acids into mitochondrial protein. Studies with oligomycin and antihistamine drugs indicate that this incorporation may not be an ATP requiring process. Several laboratories '55; Truman and Korner, '62; Bronk, '63; Kalf, '63) have reported