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Protease-Catalyzed Peptide Synthesis for the Site-Specific Incorporation of α-Fluoroalkyl Amino Acids into Peptides.

✍ Scribed by Sven Thust; Beate Koksch

Publisher
John Wiley and Sons
Year
2003
Weight
53 KB
Volume
34
Category
Article
ISSN
0931-7597

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Papain catalyzed synthesis of glyceryl esters of N-protected amino acids and peptides for the use in trypsin catalyzed peptide synthesis
✍ Yuri V. Mitin; Karin Braun; Peter Kuhl 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 113 KB 👁 2 views

Papain catalyzed synthesis of glyceryl esters of BOC(Z)-protected amino acids and peptides was performed at 40-50°C in a 50 molar excess of glycerol. Equilibrium was achieved in 6-7 h. The maximal yield of esters (50-70%) was obtained at 10% of water and pH 3.2-3.4. A lower water concentration resul