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The enzymatic synthesis of l-[3-11C]serine

✍ Scribed by H. Svärd; S.-B. Jigerius; B. Långström

Publisher
Elsevier Science
Year
1990
Weight
476 KB
Volume
41
Category
Article
ISSN
0883-2889

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✦ Synopsis

L-[3-"C]Serine has been prepared from ["Clmethanol by a series of coupled enzymatic reactions using alcoholoxidase (EC 1.1.3.13) and catalase (EC I. 1 I. 1.6) to give [" Clformaldehyde, which was condensed with tetrahydrofolate. The N,5 N'"["C]methylenetetrahydrofolate formed, was used directly with serine hydroxymethyltransferase (EC 2.1.2. I) immobilized on glutaraldehyde-activated controlled porous glass beads. L-[3-"C]Serine (specific radioactivity 30_50mCi/pmol) was obtained in a l-2% yield after a synthesis time of 5045 min counted from release of ["Clcarbon dioxide.

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