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Enzymatic synthesis and chromatographic purification of L-3-[11C]-lactic acid via D, L-3-[11C]-alanine

✍ Scribed by G. Kloster; P. Laufer

Publisher
John Wiley and Sons
Year
1980
Tongue
French
Weight
212 KB
Volume
17
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

D,L‐3‐[^11^C]‐Alanine was prepared by methylation with ^11^CH~3~I of tert butyl 2‐isocyanoacetate and subsequent hydrolysis. Enzymatic reaction using glutamate pyruvate transaminase and lactate dehydrogenase in a coupled reaction yields L‐3‐[^11^C]‐lactic acid. The radiochemical yield is 45% for D,L‐alanine and 26.5% for L‐lactic acid at a specifie activity of 0.45 mCi/μmole. Synthesis time including purification by hplc is 23 min for D,L‐3‐[^11^c]‐alanine and 45 min for L‐3‐[^11^C]‐lactic acid, respectively.

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