The effect of interferon on experimental metastases in immunocompetent and immunodeficient mice

✍ Scribed by P. Ramani; I. R. Hart; F. R. Balkwill

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: French
• Weight: 556 KB
• Volume: 37
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910370415

✦ Synopsis

A recombinant human hybrid alpha interferon (rlFN-a AID) with antiviral, immunomodulating and cell-growth-inhibitory activity on murine cells strongly inhibited the development of experimental pulrnonary metastases of the Colo 26 adenocarcinoma in BALBlc mice. Twenty-one days after i.v. injection of 5 x lo4 cells, 8/8 control mice had >200 lung tumour nodules whereas 1/6 mice receiving 500 ng rlFN-a A/ D had one lung tumour nodule and the other 5 mice were tumour-free. Equally strong inhibition was seen in immunodeficient BALBlc nulnu (athymic) and beige nu/nu (athymic and NK-deficient) mice. Scheduling experiments in vivo showed that the most important time of IFN treatment was from the day of tumour cell injection to day 5, although statistically significant reductions in lung tumour nodule number and lung weight were seen even when IFN treatment was started 7 days after cell injection. rlFN-a A/D was cytostatic to Colo 26 in vitro, causing 50% or more inhibition of cell growth or colony number at IFN levels that could be achieved in the sera of IFN-treated mice. Although rlFN-rn AID stimulated NK-cell activity in BALB/c mice, Colo 26 cells were resistant in vitro to such cells whether from control or IFN treated mice.