Prduclion oj Xicotiiiic Scid jrmir Trigoralline Nicotinic Hcntcd 10 niirra. nt 130' 30 ,. 1 9 I . 00 ,* 1 9 . . Hcntcd to 160' I , ,. 1no0 9 , 1. 218" Initinl wt. of trigoncllinc ing. .. 08.7 .. 05.4 .. 91.7 .. i8.0 . . 81.3 .. 76-3 +cr Tngoncllinc mg. 92.0 86.4 28.7 24.3 ' 0 . 8 3.0 Iicnting Nicotinic ncicl mg. 0.2 0 . 3 1.i 3.8 4.4 3.1 Yn lossof trigon ellinc (i.8 7.0 08.7 ti9.2 74.4 92.0 ncid rcfonnod (%of initinl trigonellinc) 0.2 0.3 1.0 4.8 6.4 4.1 nnd l9Oo, which is somedint lomr than the tcnipernture thnt coffee lienns might rencli during ronsting (nbout 216'). The nniount of nicotinic acid produced represents only II sinnll proportion (between 3% nud 8%) of the trigouellinc destroycct. l'yridine, in nddition to nicotinic ncid, is produced by the dcconiposition of trigonelline ; it is not produced from nicotinic ncid, ns the lntter does not decompose cvcii when licntctl itbore its melting point (2%'). THE AJIOIJST OF SICOTISIC ACID IS A Cur OF COFFEE Two snniples of coffee were csnniinetl, one I I light. ronst coffee, niid the other n (lark roiist (" :\fter Dinuer ") type. Each snniplc ivns treated ns it would lie in the prepnrntion of coffee berernge, for esaniple, by iiilding lirilf 11 pint of boiling wntcr to 9 lieaped tlesscrt-spooufiils of coffee, (i.e. 280 nil. of boiliug wiiter t o 26 g. of coffee) rilloaing it to stniitl for 8 Iiiinutcs, niid tlien decnnting and filtering the liquor. The trigonelline nnd nicotinic acid contents of tho liquor niid of the originiil coffee were determined. (Tiibles IIIA nnd HID). T'\IlLC IIZA Brlnreliorr of l'riyoirtlliiie frorii Rmalul Coffec ~bTrigoncllinc Trit.ouclline in liquor (l0yb clccoction) in colfce n~g./lW nil. ?& of co1li.e cvtrnction Xoniinl ronst 0.05 ti1 -3 0.02 92-4 Dnrk ronst 0.40 44-7 0.46 93.9 TmtE IIIil Er/rcirfioii oj Sicofiiiic .-kid jrorri noasled Coffee Sicctinic ncicl in liquor (lo?& decoction) In co(Fecpg./g. nig./lOO ml. pg./g. ofcoffcc % cstrnction Sorninl mnst 110 1 *o 10 1 91.8 Dnrk ronst "02 1 . 0 1 D : ! OZ.0 A cup of wliite coffec (200 nil., linlf loo/, coffee decoction nnd linlf inilk) niiglit tliercforc coiitnin bctaecn 1 i l n d 2 ~n g . of nicotinic ncid, neeording to the tilie of coffee used. Tlicsc results m e siniilnr t o tliosc olitniiicd by Teply, Drelil niid Elrelijein,ll who foulid tliiit cups of cofrec purclinsed a t fire differcut restnuriitits nrernged 1 ing. of nicotinic ncitl per cup (175 xnl.).
ACIZSO\'LEnoE~IENTS
The dctcrininntions of nicotinic acid were iiinde by Mr. T. L.
Piirkinson. Tlie authors wish to thnnk the directors of J. Lyons 6r Co. Ltcl. for their perniission to publish the results of this inrestigntion.