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The determination of free ε-amino groups in proteins

✍ Scribed by Oratz, Murray; Burks, Alvin L.; Rothschild, Marcus A.

Book ID
122994586
Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
548 KB
Volume
115
Category
Article
ISSN
0304-4165

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The determination of free ϵ-amino groups of lysine in proteins using 19F NMR spectroscopy
✍ J.E. Ramirez; J.R. Cavanaugh; K.S. Schweizer; P.D. Hoagland 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 301 KB

The amount of free e-amino groups of lysine residues in proteins can be determined by a fast and straightforward method based on the use of r9F NMR spectroscopy. Unsubstituted e-amino groups are trifluoroacetylated with the reagent, S-ethyl trifluorothioacetate, in dimethyl sulfoxide solution, and t