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The determination of free ϵ-amino groups of lysine in proteins using 19F NMR spectroscopy

✍ Scribed by J.E. Ramirez; J.R. Cavanaugh; K.S. Schweizer; P.D. Hoagland

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 301 KB
Volume: 63
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90196-7

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✦ Synopsis

The amount of free e-amino groups of lysine residues in proteins can be determined by a fast and straightforward method based on the use of r9F NMR spectroscopy. Unsubstituted e-amino groups are trifluoroacetylated with the reagent, S-ethyl trifluorothioacetate, in dimethyl sulfoxide solution, and the number of such groups is quantitatively determined using standard 19F NMR techniques. The method is evaluated using the following proteins: a-lactalbumin, P-lactoglobulin, bovine serum albumin, p-casein, and lysozyme.

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