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Determination of ε-acetimidyllysine in proteins

✍ Scribed by Bryce V. Plapp; Jack C. Kim

Book ID
107711622
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
210 KB
Volume
62
Category
Article
ISSN
0003-2697

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The determination of free ϵ-amino groups of lysine in proteins using 19F NMR spectroscopy
✍ J.E. Ramirez; J.R. Cavanaugh; K.S. Schweizer; P.D. Hoagland 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 301 KB

The amount of free e-amino groups of lysine residues in proteins can be determined by a fast and straightforward method based on the use of r9F NMR spectroscopy. Unsubstituted e-amino groups are trifluoroacetylated with the reagent, S-ethyl trifluorothioacetate, in dimethyl sulfoxide solution, and t