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The construction in vitro of transducing derivatives of phage lambda

✍ Scribed by Borck, Kathleen ;Beggs, Jean D. ;Brammar, W. J. ;Hopkins, A. S. ;Murray, Noreen E.

Publisher: Springer
Year: 1976
Tongue: English
Weight: 968 KB
Volume: 146
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00268089

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✦ Synopsis

Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.

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