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The isolation and genetic characteristics of lambda transducing phages of the uvrA + and uvrC + genes of E. coli K12

✍ Scribed by Auerbach, Jeffrey ;Howard-Flanders, Paul

Publisher: Springer
Year: 1979
Tongue: English
Weight: 334 KB
Volume: 168
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00271506

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✦ Synopsis

Lambda transducing phages carrying the excision repair genes uvrA+ and uvrC+ were selected from a pool of lambda phages carrying EcoR1 fragments of E. coli DNA. These phages and also lambdauvrB+ (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA-, uvrB- or uvrC-. Lambda uvrA+ was found to transduce strains carrying uvrA- but not those carrying uvrB- or uvrC-, to normal ultraviolet resistance. Similarly, lambdauvrB+ and lambdauvrC+ were found to complement only the corresponding uvr- allele. The lambda transducing phages were co-transduced with gal+ by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site.

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