W-reactivation of phage lambda in recF, recL, uvrA, and uvrB mutants of E. coli K-12

✍ Scribed by Rothman, Robert H. ;Margossian, Linda J. ;Clark, Alvin J.

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 850 KB
• Volume: 169
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00382274

✦ Synopsis

W-reactivation is reduced by recF143 and recF144 mutations and is undetectable if a second mutation at either the uvrA or uvrB locus is combined with recF143. The uvrA and uvrB mutations alone block W-reactivation partially. A reeL152 mutation also partially blocks W-reactivation by itself. In combination with a uvrB5 mutation, recL125 blocks W-reactivation completely but in combination with recFI43, significant residual W-reactivation ability remains. We suggest that the phenomenon of W-reactivation is the result of at least two modes or pathways. The observation that recF143 uvrB5 and recFI43 uvrA6 strains permit normal levels of mutagenesis (Kato et al., 1977) but completely block all W-reactivation leads us to suggest further that the mechanism(s) of W-reactivation is at least partly different from that of UV mutagenesis.