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Localization of the metJBLF gene cluster of Escherichia coli in λmet transducing phage

✍ Scribed by Greene, Ronald C. ;Krueger, Judy H. ;Johnson, James R.

Publisher
Springer
Year
1982
Tongue
English
Weight
547 KB
Volume
187
Category
Article
ISSN
0026-8925

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✦ Synopsis

The position of the metJBLF gene cluster in the transducing phage lambda met102 was determined by ligation of its leftmost EcoRI fragment (102-1) to the lambda BCDEF (nin5) EcoRI fragment of lambda gtl (lambda BC) and characterization of the resultant recombinant phage. The new transducing phage carries about 6kb of bacterial DNA which contains the entire met gene cluster including the promoter of its rightmost member metF. Reasonable estimates of the coding capacity required for the four genes indicate that most of the bacterial DNA of the recombinant phage is occupied by the met gene cluster.

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