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A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing λdilv and λdrbs phages

✍ Scribed by Jørgensen, Poul ;Collins, John ;Fiil, Niels ;von Meyenburg, Kaspar

Publisher
Springer
Year
1978
Tongue
English
Weight
594 KB
Volume
163
Category
Article
ISSN
0026-8925

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✦ Synopsis

Specialized transducing phages carrying segments of the Escherichia coli chromosome from the rbs-ilv region including rrn genes have been isolated. These phages carry rrn transcription units coding for 16S and 23S rRNA with the direction of transcription clockwise towards the ilv operon. While one of the phages (lambdad279rbs) appears to carry the genuine rrn gene, denoted rrnC, located between rbs and ilv at 82 min on the E. coli chromosome another one isolated as an ilv transducing phage, lambda5ilv, carries a hybrid rrn gene, denoted rrnX, which has originated from a recombinational cross-over between the rrnC and one of the other rrn genes.

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