✦ LIBER ✦

The action of cytochalasin A on the in vitro polymerization of brain tubulin and muscle G-actin

✍ Scribed by Himes, Richard H. ;Houston, L. L.

Publisher: Wiley (John Wiley & Sons)
Year: 1976
Tongue: English
Weight: 454 KB
Volume: 5
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400050109

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The presence of cytochalasin A inhibits the self‐assembly of beef brain tubulin and rabbit muscle G‐actin in vitro and also decreases the colchicine binding of tubulin. Prior reaction of cytochalasin A with 2‐mercaptoethanol destroys its inhibitory effects. It is shown that cytochalasin A exerts its actions by reacting with sulfhydryl groups, possibly causing irreversible structural changes in the proteins. Cytochalasin B does not affect the tubulin assembly reaction.

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