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In vivo and in vitro effect of cold on the degree of polymerization of tubulin in toad brain and testis

✍ Scribed by Lopez, Luis A. ;Bertini, Francisco

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
346 KB
Volume
221
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The tubulin of both brain and testis of the toad Bufo arenarum was partially depolymerized when the animals were maintained on ice for 90 minutes or 17 hours. Recovery of the animals at room temperature restored the normal degree of polymerization in both organs. Normalization of the polymerization degree also occurred in organs separated from cooled animals and incubated in vitro for 90 minutes at 20°C. When the organs were cooled in vitro, only testicular tubulin underwent depolymerization, and in a degree similar to that occurring by incubation at 20°C. The results suggest that the mechanisms regulating tubulin depolymerization in vivo are different from those taking place in vitro.

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