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The 9-fluorenylmethyloxycarbonyl group as a 5′-OH protection in oligonucleotide synthesis

✍ Scribed by Yunxi Ma; Etienne Sonveaux

Publisher
Wiley (John Wiley & Sons)
Year
1989
Tongue
English
Weight
521 KB
Volume
28
Category
Article
ISSN
0006-3525

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✦ Synopsis

Oligo-DNAs are synthesized on a solid support using the 9-fluorenylmethyloxycarbonyl group as a 5'-OH base labile protection. The synthesis of the pure protected nucleotides, a relevant phosphoramidite-type strategy of coupling, and the optimization of the deprotection steps are described. This new synthetic method i s an alternative to the standard protocol that avoids acidic conditions.

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Synthesis of pentathymidylate using a 4-
Synthesis of pentathymidylate using a 4-monomethoxytritylthio (MMTrS) group as a 5′-hydroxyl protecting group: toward oligonucleotide synthesis without acid treatment
✍ Kohji Seio; Mitsuo Sekine 📂 Article 📅 2001 🏛 Elsevier Science 🌐 French ⚖ 82 KB

A phosphoramidite unit having 4-monomethoxytritylthio as a new 5%-hydroxyl protecting group was prepared and employed in oligonucleotide synthesis. The new phosphoramidite enabled the synthesis of oligonucleotides without the use of acids such as TFA or DCA.