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The 5-methylcytosine content of DNA: Tissue specificity

✍ Scribed by John W. Kappler

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
317 KB
Volume
78
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Using a new technique for the determination of the extent of DNA methylation by comparing the incorporation of radioactivity from deoxycytidine‐2‐C^14^ into DNA cytosine and 5‐methylcytosine (5MC), the 5MC content of DNA was found to be a tissue specific property in two systems: (1) mouse tissue culture cell lines, in which the level of 5MC varied from 2.97 Β± 0.03% conversion of cytosine to 5MC in the DNA of a mouse melanoma line to 4.58 Β± 0.18% in a primary teratoma line, and (2) the organs of the developing chick embryo, in which the level of 5MC varied from 3.60 Β± 0.04% conversion of cytosine to 5MC in the DNA of the chorioallantoic membrane to 4.19 Β± 0.02% in the brain.

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