Separation of 5-methylcytosine-rich DNA using immobilized antibody

Sensitive detection of 5-methylcytosine

Sensitive detection of 5-methylcytosine and quantitation of the 5-methylcytosine/cytosine ratio in DNA by gas chromatography-mass spectrometry using multiple specific ion monitoring

✍ Judith Singer; William C. Schnute Jr.; John E. Shively; Charles W. Todd; Arthur 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 320 KB

A method combining gas chromatography and mass spectrometry (GC-MS) with multiple specific ion monitoring has been developed for the detection of S-methylcytosine and the quantitation of the ratio of methylcytosine to cytosine in DNA. The trimethylsilyl derivatives of cytosine and S-methylcytosine o