𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective Detection of 5-Methylcytosine Sites in DNA

✍ Scribed by Sébastian Bareyt; Thomas Carell


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
361 KB
Volume
47
Category
Article
ISSN
0044-8249

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Analysis of 5-methylcytosine in DNA: I.
✍ Joseph Deutsch; Aharon Razin; John Sedat 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 419 KB

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in +x 174 DNA, in a sample of less than 10 pg and requires les

Analysis of 5-methylcytosine in DNA: II.
✍ Aharon Razin; John Sedat 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 454 KB

A method for analyzing 5methylcytosine in DNA by gas chromatography is described. The method is based on degradation of the DNA to its free bases by treatment with trifluoroacetic acid and gas chromatography of the trimethylsilyl derivatives of the free bases. Chromatography of microgram amounts of

The 5-methylcytosine content of DNA: Tis
✍ John W. Kappler 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 English ⚖ 317 KB

## Abstract Using a new technique for the determination of the extent of DNA methylation by comparing the incorporation of radioactivity from deoxycytidine‐2‐C^14^ into DNA cytosine and 5‐methylcytosine (5MC), the 5MC content of DNA was found to be a tissue specific property in two systems: (1) mou

Sensitive detection of 5-methylcytosine
✍ Judith Singer; William C. Schnute Jr.; John E. Shively; Charles W. Todd; Arthur 📂 Article 📅 1979 🏛 Elsevier Science 🌐 English ⚖ 320 KB

A method combining gas chromatography and mass spectrometry (GC-MS) with multiple specific ion monitoring has been developed for the detection of S-methylcytosine and the quantitation of the ratio of methylcytosine to cytosine in DNA. The trimethylsilyl derivatives of cytosine and S-methylcytosine o