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Analysis of 5-methylcytosine in DNA: I. Mass spectrometry

✍ Scribed by Joseph Deutsch; Aharon Razin; John Sedat

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
419 KB
Volume
72
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in +x 174 DNA, in a sample of less than 10 pg and requires less than 1 pg of calf thymus DNA in which the molar ratio of 5-methylcytosine/ cytosine is about 0.05.

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