𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Analysis of Uracil in DNA by Gas Chromatography-Mass Spectrometry

✍ Scribed by B.C. Blount; B.N. Ames

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
539 KB
Volume
219
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A sensitive method using gas chromatography and negative chemical ionization mass spectrometry for the detection of uracil in DNA is described. Uracil DNA glycosylase is used to specifically cleave uracil from DNA. Once removed, uracil is derivatized with 3,5-bis(trifluoromethyl)benzyl bromide and the sample components are separated with gas chromatography. Negative chemical ionization mass spectrometry is used to quantitatively detect as little as 1 pg of uracil per 100 micrograms DNA. This assay is 10 times more sensitive than previously described quantitative methods for the detection of uracil in DNA. We apply this method as part of a project to determine why folic acid deficiency causes DNA breaks. We demonstrate that inhibition of folic acid metabolism induces an accumulation of uracil in DNA.

πŸ“œ SIMILAR VOLUMES

Analysis of fragrances in cosmetics by g
Analysis of fragrances in cosmetics by gas chromatography-mass spectrometry
✍ Rastogi, Suresh Chandra πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 579 KB

## Abstract A gas chromatographic (GC)‐mass spectrometric (GC‐MS) method has been developed for the routine analysis of 11 fragrance substances in cosmetics: cinnamic alcohol, cinnamic aldehyde, eugenol, hydroxy citronellal, α‐amyl cinnamic aldehyde, geraniol, isoeugenol, coumarin, dihydrocoumarin,

Analysis of hydroxypipecolic acids by ga
Analysis of hydroxypipecolic acids by gas chromatography–mass spectrometry
✍ Geoffrey C. Kite; Michael J. Hughes πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 158 KB πŸ‘ 2 views

The development of a method is described for the analysis of hydroxypipecolic acids in crudely purified plant extracts. All eight known naturally-occurring mono-and di-hydroxypipecolic acids could be resolved in a single analysis by gas chromatography-mass spectrometry (GC-MS) of their trimethylsily

Analysis of flavonoids in tablets and ur
Analysis of flavonoids in tablets and urine by gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry
✍ D. G. Watson; A. R. Pitt πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 91 KB πŸ‘ 2 views

A method was developed for the analysis and characterization of quercetin and kaempferol in urine following ingestion of Ginkgo biloba tablets. The method utilized gas chromatography/negative ion chemical ionization mass spectrometry of the trimethysilyl derivatives of the flavonoids. Limits of dete

Analysis of alkenes by copper ion chemic
Analysis of alkenes by copper ion chemical ionization gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry
✍ Fordham, P. J.; Chamot-Rooke, J.; Giudice, E.; Tortajada, J.; Morizur, J.-P. πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 164 KB πŸ‘ 2 views

A novel chemical ionization/fast atom bombardment (CI/FAB) source was used to analyse alkenes by chemical ionization mass spectrometry (CI-MS) using copper ions as the ionizing agent. The Cu Y -CI mass spectra showed abundant pseudomolecular adduct ions [alkene-Cu] Y and characteristic fragment ions