A sensitive method using gas chromatography and negative chemical ionization mass spectrometry for the detection of uracil in DNA is described. Uracil DNA glycosylase is used to specifically cleave uracil from DNA. Once removed, uracil is derivatized with 3,5-bis(trifluoromethyl)benzyl bromide and t
Analysis of fragrances in cosmetics by gas chromatography-mass spectrometry
β Scribed by Rastogi, Suresh Chandra
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 579 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
A gas chromatographic (GC)βmass spectrometric (GCβMS) method has been developed for the routine analysis of 11 fragrance substances in cosmetics: cinnamic alcohol, cinnamic aldehyde, eugenol, hydroxy citronellal, Ξ±βamyl cinnamic aldehyde, geraniol, isoeugenol, coumarin, dihydrocoumarin, citronellal and citral. Methods for sample preparation of various types of cosmetic products, prior to GC analysis, have also been developed and proved to be rugged. Detection limits of all of target fragrance substances were approximately 1 ppm. Calibration curves of the target fragrance substances analyzed by GC were found to be linear in the investigated concentration range, 0.005% β 0.50%. The recoveries of the target fragrances from various types of cosmetic products were 80% β 116% and the relative standard deviations of the quantitative analysis of the target fragrance substances were within 5%.
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