We have previously demonstrated systemic resistance to methotrexate (MTX) in transgenic mice carrying a foreign, mutant dihydrofolate reductase (DHFR, E.C. 1 S.1.3) gene. The new gene was introduced as a cDNA cloned into an expression vector driven by the simian virus 40 (SV40) early promoter. Previ
Systemic Resistance to Methotrexate in Transgenic Mice Carrying a Mutant Dihydrofolate Reductase Gene
โ Scribed by Luis M. Isola and Jon W. Gordon
- Book ID
- 123633670
- Publisher
- National Academy of Sciences
- Year
- 1986
- Tongue
- English
- Weight
- 787 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/28675
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๐ SIMILAR VOLUMES
Several lines of transgenic mice were produced by pronuclear injection of a full-length cDNA encoding a mutant dihydrofolate reductase (DHFR, E.C. 1.5.1.3). The mutation causes altered enzyme kinetics for folate reduction as well as low affinity for methotrexate (MTX). One line of mice carrying the
Dihydrofolate reductase was synthesized in a batch system in the presence of the af6Nty ligand methotrexate, bound to various matrices. l k o types of gel were used: commercial methotrexate-agarose with pores inaccessible for translation machinery and methotrexate-POROS with pores easily accessible