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Synthesis of β-Mannosides via Prearranged Glycosides

✍ Scribed by Thomas Ziegler; Gregor Lemanski


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
120 KB
Volume
37
Category
Article
ISSN
0044-8249

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β-Mannosynthase: Synthesis of β-Mannosid
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Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized. Remarkably, α-mannos