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β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase

✍ Scribed by Oyekanmi Nashiru; David L. Zechel; Dominik Stoll; Taraneh Mohammadzadeh; R. Antony J. Warren; Stephen G. Withers

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
175 KB
Volume
113
Category
Article
ISSN
0044-8249

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β-Mannosynthase: Synthesis of β-Mannosid
β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase
✍ Oyekanmi Nashiru; David L. Zechel; Dominik Stoll; Taraneh Mohammadzadeh; R. Anto 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 176 KB 👁 1 views

Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized. Remarkably, α-mannos