β-Mannosynthase: Synthesis of β-Mannosides with a Mutant β-Mannosidase

✍ Scribed by Oyekanmi Nashiru; David L. Zechel; Dominik Stoll; Taraneh Mohammadzadeh; R. Antony J. Warren; Stephen G. Withers

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 176 KB
• Volume: 40
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/1521-3773(20010119)40:2<417::aid-anie417>3.0.co;2-v

✦ Synopsis

Engineering enzymes: The glutamic acid nucleophile of a retaining β-mannosidase has been replaced with a serine residue to form a β-mannosynthase. When the new enzyme is provided with an α-mannosyl fluoride donor and an appropriate acceptor, β-mannoside linkages are synthesized. Remarkably, α-mannosyl fluoride can be generated in situ by providing the mannosynthase with excess fluoride ion.