Synthesis of TMG-capped RNA–DNA chimeric oligonucleotides

✍ Scribed by Mitsuo Sekine; Masatoshi Ushioda; Takeshi Wada; Kohji Seio

• Book ID: 104252889
• Publisher: Elsevier Science
• Year: 2003
• Tongue: French
• Weight: 153 KB
• Volume: 44
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(02)02828-9

✦ Synopsis

This paper deals with the synthesis of m 3 2,2,7 G 5% pppAmpUmpApd(CpTpTpApCpCpT), a 5%-TMG-capped RNA-DNA chimeric oligonucleotide, which is expected to be conveyed to the nucleus by snurportin 1, a nuclear-transport protein. This 5%-TMG-capped RNA-DNA chimeric molecule was synthesized by the enzymatic condensation of m 3 2,2,7 G 5% pppAmpUmpA with A 5% ppd(CpTpTpApCpCpT) in the presence of RNA ligase. An inherent serious side reaction was disclosed in the 5%-adenylation of d(CpTpTpApCpCpT) on the solid support, but the use of an active ester intermediate as the pA donor gave an improved result.