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Synthesis of sulfur-35 reagents for protein labeling

✍ Scribed by Sumei Ren; Paul McNamara; David Koharski; David Hesk; Scott Borges

Publisher
John Wiley and Sons
Year
2007
Tongue
French
Weight
101 KB
Volume
50
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

Two ^35^S reagents were developed to radiolabel proteins. The first reagent, a N‐hydroxysuccinimide (NHS) ester (SMSB), acylates the ε‐amino group of lysine residues in proteins. The second reagent, an aldehyde (MSAPPA), labels lysine residues via reductive alkylation. Comparing the two methods, the reductive alkylation method labeled proteins over a broader pH range with higher overall radiochemical yield. The biological activity of the proteins did not change after labeling with these ^35^S reagents. Copyright © 2007 John Wiley & Sons, Ltd.

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