✦ LIBER ✦
Suitability of sulfur labelling reagent (Amersham) as an in vitro protein radiolabelling reagent for two-dimensional electrophoresis
✍ Scribed by Dr. Thierry Rabilloud; HéLène Therre
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 335 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Suitability of sulfur labelling reagent (Amersham) as an in vitro protein radiolabelling reagent for two-dimensional electrophoresis
This report describes a method of in vitro labelling ofproteins designed for subsequent analysis of the sample by two-dimensional electrophoresis. As much as lo6 cpm can be incorporated in 5 pg of proteins. As the reagent reacts with the amino groups, virtually all the proteins are labelled. Thus, this method is far more sensitive than silver staining and may prove useful when extremely low amounts of sample are to be analy sed.