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Synthesis of S-adenosyl-L-homocysteine and 5′-methylthioadenosine specifically tritiated at the 5′C position

✍ Scribed by G. Guillerm; M. C. Galas; F. Le Goffic; J. P. Beaucourt; L. Sergent

Publisher: John Wiley and Sons
Year: 1986
Tongue: French
Weight: 258 KB
Volume: 23
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2580230807

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✦ Synopsis

S-adenosyl-L-homocysteine (AdoHcy) is an important metabolic product in a variety of processes (1.2). This compound as well as 5'-methylthioadenosine are substrates for AdoHcy nucleosidase. an enzyme required In various prokaryotes cells (3.4.5.6) for the recovery of the enzymatic activity of methylases through the splitting of S-adenosylhomocystelne.

For the evaluation of inhibitors of AdoHcy nucleosidase ve developped ( 7 ) a more simple and reliable enzymatic assay than those previously described (3-8).

It uses specifically tritiated AdoHcy

o r 5'-methylthioadenosine vhich are split during the enzymatic reaction into adenine and ' H S-ribosyl L-homocysteine or ' H 5'-methylthioribose respectively ( 3 ) .

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