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Synthesis of Oligonucleotide Derivatives Using ChemMatrix Supports

✍ Scribed by Stefania Mazzini; Fayna García-Martin; Margarita Alvira; Anna Aviñó; Brendan Manning; Fernando Albericio; Ramon Eritja

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
372 KB
Volume
5
Category
Article
ISSN
1612-1872

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✦ Synopsis

Abstract

The synthesis of oligonucleotides on poly(ethylene glycol)‐based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthesis of oligonucleotide–peptide conjugates and for the ligation of oligonucleotides using Cu^+^‐catalyzed cycloadition reactions is reported. Moreover, these supports can be used for the preparation of oligonucleotides in anhydrous solvents, followed by hybridization of the complementary sequences in aqueous buffers.

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## Abstract Oligonucleotides containing terminal phosphate groups serve as useful intermediates for different applications in molecular and cell biology, as well as for diagnostic purposes. The chemical synthesis of these derivatives has been an important topic of oligonucleotide research. We repor