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Synthesis of Oligonucleotides by Use of Phosphoric Triesters

✍ Scribed by Dr. F. Eckstein; Dipl.-Chem I. Rizk

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
204 KB
Volume
6
Category
Article
ISSN
0044-8249

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**The mixture of homologous nucleotide fragments** usually formed during carrier‐oligonucleotide synthesis can be separated chromatographically so as to isolate the desired fragment, if the latter is protected at the 5′‐end with a hexadecyloxytrityl group (see __(2)__ in the previous communication).

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## Abstract The synthesis of oligonucleotides on poly(ethylene glycol)‐based (ChemMatrix) supports was studied. Results show that oligonucleotides can be indeed prepared in good yields using slightly modified synthesis cycles and automated DNA synthesizers. The use of these supports for the synthes