𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis of a (+)-anatoxin-a analogue for monoclonal antibodies production

✍ Scribed by Mickael Marc; Francis Outurquin; Pierre-Yves Renard; Christophe Créminon; Xavier Franck

Publisher
Elsevier Science
Year
2009
Tongue
French
Weight
312 KB
Volume
50
Category
Article
ISSN
0040-4039

No coin nor oath required. For personal study only.

✦ Synopsis

Anatoxin-a analogue was prepared by resolution of aminoacid 1 using chiral (R)-4-phenyl-oxazolidin-2thione as derivatizing agent. X-ray diffraction of a diastereomer allowed us to determine its absolute configuration. The synthesis could then be completed in few steps followed by introduction of an amide linker bearing a terminal alkyne in order to attach a carrier protein for monoclonal antibodies production.

📜 SIMILAR VOLUMES

Synthesis of (±)-Anatoxin-a and Analogue
Synthesis of (±)-Anatoxin-a and Analogues
✍ Philip J Parsons; Nicholas P Camp; Neil Edwards; L Ravi Sumoreeah 📂 Article 📅 2000 🏛 Elsevier Science 🌐 French ⚖ 160 KB
Production of monoclonal antibodies agai
Production of monoclonal antibodies against a cell surface concanavalin a binding glycoprotein
✍ Starling, James J. ;Simrell, Charles R. ;Klein, Paul A. ;Noonan, Kenneth D. 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 908 KB

Concanavalin A-binding (Con A)-binding cell surface glycoproteins were isolated, via Con A-affinity chromatography, from Triton X-1 00-solubilized Chinese hamster ovary (CHO) cell plasma membranes. The Con A binding glycoproteins isolated in this manner displayed a significantly different profile on

Synthesis of a non-hydrolyzable AZT-trip
Synthesis of a non-hydrolyzable AZT-triphosphate analogue designed for the production of anti-AZT-TP antibodies
✍ Thierry Brossette; Alain Valleix; Laure Goujon; Christophe Cre´minon; Jacques Gr 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 261 KB

An enzymatically stable analogue of AZT-triphosphate is prepared in 7 steps starting from thymidine. The thymine moiety is thnctionalized at N 3 to allow the further cross-linking to a carrier protein for subsequent immunization of rabbits in order to raise specific antibodies directed against AZT-T

A novel method for monitoring monoclonal
A novel method for monitoring monoclonal antibody production during cell culture
✍ Henryk Szmacinski; Derek S. Smith; Michael A. Hanson; Yordan Kostov; Joseph R. L 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 465 KB

## Abstract We describe a new format for surface‐based fluoroimmunoassays that allows detection of biomolecule interactions without separation steps. The bioactive layer was immobilized on the surface of a glass substrate covered with silver islands that provide optical amplification of the distinc

Production of a diagnostic monoclonal an
Production of a diagnostic monoclonal antibody in perennial alfalfa plants
✍ Habib Khoudi; Serge Laberge; Jean-Marc Ferullo; Renée Bazin; André Darveau; Yves 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 249 KB

The increasing use of monoclonal antibodies (mAbs) in diagnostic reagents necessitates efficient and cost-effective mAb production methods. In blood banks, one of the most routinely used reagents is the antihuman IgG reagent used for the detection of nonagglutinating antibodies. Here we report the p