𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A novel method for monitoring monoclonal antibody production during cell culture

✍ Scribed by Henryk Szmacinski; Derek S. Smith; Michael A. Hanson; Yordan Kostov; Joseph R. Lakowicz; Govind Rao

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
465 KB
Volume
100
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

We describe a new format for surface‐based fluoroimmunoassays that allows detection of biomolecule interactions without separation steps. The bioactive layer was immobilized on the surface of a glass substrate covered with silver islands that provide optical amplification of the distinctive fluorescence signal from bound probes when compared to unbound probes. The technique used was phase‐modulation fluorometry that allows sensitive detection of bound probes with a very short lifetime in the presence of excess free probes in solution. The new method was applied to assay monoclonal antibody production during cell culture. Excellent agreement was found between the new method and ELISA analysis of hybridoma cell culture samples. It is predicted that the near real time monitoring of protein products during bioprocessing will be possible with the described technology. Biotechnol. Bioeng. 2008;100: 448–457. Β© 2008 Wiley Periodicals, Inc.

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