On-line monitoring of monoclonal antibodies in animal cell culture using a grating coupler
✍ Scribed by R. Polzius; F. F. Bier; U. Bilitewski; V. Jäger; R. D. Schmid
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 537 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A flow injection analysis (FIA) biosensor system has been developed for on-line determination of glucose during mammalian cell cultivation. The culture sample was peristaltically withdrawn from the bioreactor and after cell separation by a steam sterilizable ceramic microfilter, the filtrate was con
The use of on-line relative permittivity (Ј) measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing -galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in Ј in bo
On-line, "real-time" monitoring of product concentration is important for mammalian cell culture fermentation. The continuous measurement of monoclonal antibodies allows for instantaneous determination of cell productivity and effective manipulation of the fermentor operating conditions for optimal