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On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements

✍ Scribed by A. Zeiser; C. Bédard; R. Voyer; B. Jardin; R. Tom; A. A. Kamen

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 101 KB
Volume: 63
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19990405)63:1<122::aid-bit13>3.0.co;2-i

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✦ Synopsis

The use of on-line relative permittivity (Ј) measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing ␤-galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in Ј in both non-infected and synchronously infected cultures, indicating that the Ј of a cell suspension was sensitive only to changes in the viable cell population. Additionally the parameter provided clearly defined signposts of the progress of the infection.

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