Phosphate feeding improves high-cell-concentration NS0 myeloma culture performance for monoclonal antibody production

Fed-batch culture of recombinant NS0 mye

Fed-batch culture of recombinant NS0 myeloma cells with high monoclonal antibody production

✍ Weichang Zhou; Chun-Chiang Chen; Barry Buckland; John Aunins 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 231 KB 👁 2 views

An amplified NS0 cell line transfected with a vector expressing a humanized monoclonal antibody (MAb) against CD-18 and glutamine synthetase (GS) was cultivated in a 1.5 L fed-batch culture using a serum-free, glutamine-free medium. Concentrated solutions of key nutrient components were fed periodic