𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis of 5′-phosphorylated DNA fragments

✍ Scribed by G. van der Marel; G. Veeneman; J.H. van Boom

Book ID
104241546
Publisher
Elsevier Science
Year
1981
Tongue
French
Weight
284 KB
Volume
22
Category
Article
ISSN
0040-4039

No coin nor oath required. For personal study only.

✦ Synopsis

Morpholino phosphorobis-3-nitro-1,2,4_triazolidate (L) has been applied to the phosphorylation of partially protected (5'-OH free) DNA intermediates (2). Using this procedure, the following types of compounds were synthesized: ~B~(BP)~B; APPBP(BP)~B and PPBP(BP),B. The synthesis of DNA fragments (i.e. 7-'; R1=R2=R3=H; B=T,C,A or G) with a defined sequence 1) and length via phosphotriester intermediates is a well established technique . In a strategy based on the phosphotriester approach to the synthesis of DNA fragments, we may discern two distinct stages. The first one involves the synthesis of a fully-protected intermediate (i.e.

📜 SIMILAR VOLUMES

Synthesis of DNA fragments containing 5-
Synthesis of DNA fragments containing 5-methyl-deoxycytidine residues
✍ G. A. van der Marel; G. Wille; H. Westerink; A. H.-J. Wang; A. Rich; J. R. Melle 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 195 KB

## Abstract A properly protected 5‐methylated deoxycytidine (5mC) derivative has been synthesized and applied to assemble DNA fragments containing 5mCG, 5mCC and 5mC5mC sequences.

Synthesis and enzymatic incorporation of
Synthesis and enzymatic incorporation of morpholino thymidine-5′-triphosphate in DNA fragments
✍ Florence Marciacq; Sylvie Sauvaigo; Jean-Paul Issartel; Jean-François Mouret; Di 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 257 KB

4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydro~methyl)morpholine~-triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1 -(PD-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide anal