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Synthesis and enzymatic incorporation of morpholino thymidine-5′-triphosphate in DNA fragments

✍ Scribed by Florence Marciacq; Sylvie Sauvaigo; Jean-Paul Issartel; Jean-François Mouret; Didier Molko

Book ID
104261625
Publisher
Elsevier Science
Year
1999
Tongue
French
Weight
257 KB
Volume
40
Category
Article
ISSN
0040-4039

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✦ Synopsis

4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydro~methyl)morpholine~-triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1 -(PD-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide analogue into DNA fragments was investigated. Morpholino thymidine-5'-triphosphate was incorporated in a base-specific process and acted as a novel chain terminator in DNA sequencing, similarly to the corresponding dideoxynucleotide.

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An enzymatic procedure for the synthesis
An enzymatic procedure for the synthesis of 3H-thymidine-5′-triphosphate
✍ Brunhild Preussel; Marion Fritz; Peter Lange 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 French ⚖ 290 KB 👁 1 views

## Abstract An enzymatic method for synthesis of tritium‐labelled thymidine‐5′‐triphosphate is described. The procedure utilizes a nucleoside phosphotransferase from wheat germ and a partially purified thymidylate kinase from calf thymus. The product is isolated by ion‐exchange chromatography on DE