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An enzymatic procedure for the synthesis of 3H-thymidine-5′-triphosphate

✍ Scribed by Brunhild Preussel; Marion Fritz; Peter Lange

Publisher: John Wiley and Sons
Year: 1978
Tongue: French
Weight: 290 KB
Volume: 14
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2580140418

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✦ Synopsis

Abstract

An enzymatic method for synthesis of tritium‐labelled thymidine‐5′‐triphosphate is described. The procedure utilizes a nucleoside phosphotransferase from wheat germ and a partially purified thymidylate kinase from calf thymus. The product is isolated by ion‐exchange chromatography on DEAE‐cellulose and can be obtained in a yield of approximately 40% and at a radiochemical purity of 95%.

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